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You are here:Home » Antibodies » Abs to IgG Kappa » Anti -Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809)

Anti -Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa
Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km,
MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
Immunohistochemical detection of immunoglobulins is useful in determining the lymphoid origin of tissues and in the assessment of lymphoid proliferations as reactive or malignant. The assessment of light chain status is particularly important in this regard as it may be used to demonstrate the clonal nature of a tumor.
Catalog #K0080
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (frozen and formalin/paraffin): 1-2ug/ml. for 30min at RT.
Note: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlTonsil
Cellular LocalizationCytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.343
HostMouse
SourceHuman
Concentration0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.
Also available without BSA and azide. See K0080X
PurityPurified by Protein G affinity chromatography.
ImmunogenB lymphoma cells.
SpecificityRecognizes the kappa light chain of immunoglobulin.
Species CrossreactivityHuman. Does not react with Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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