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You are here:Home » Antibodies » Antibodies-IgG Kappa Conjugates » Anti -Kappa Chain (B Cells) (Biotin)

Anti -Kappa Chain (B Cells) (Biotin)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
Human immunoglobulins are glycoproteins composed of two disulfide-bonded heavy (H) chain subunits, each of which is linked by interchain disulfide bonds to a light (L) chain forming a tetramolecular complex. There are five classes of immunoglobulins, designated IgG, IgA, IgM, IgD and IgE, which are defined by differences in the constant region of H chains. L chains are divided into kappa or lambda classifications based on structural antigenic differences. All classes of immunoglobulins have been found on the cell surface of B lymphocytes where they function as antigen receptors to elicit antigen-dependent proliferation and secretion of antigen specific soluble circulating antibodies.
Catalog #K0080-06B
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. 5x10e5 ficoll prepared human peripheral white blood cells were preincubated ~5 minutes with 20ul of 250ug/ml human Ig after which they were incubated 45 minutes on ice with 80ml of K0080-06B at 10ug/ml.
2. Cells were washed twice and incubated with 2º reagent Streptavidin/R-PE (S7973-88P) after which they were washed three times, fixed and analyzed using a BD FACS Calibur.
3. Cells stained positive with a mean shift of 2.17 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration.
4. Binding was 99% blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml anti-Kappa antibody (K0080-06).
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No7H201
FormSupplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with Biotin.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman B lymphoma cells
SpecificityRecognizes cell surface kappa on human B cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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