| Human immunoglobulins are glycoproteins composed of two disulfide-bonded heavy (H) chain subunits, each of which is linked by interchain disulfide bonds to a light (L) chain forming a tetramolecular complex. There are five classes of immunoglobulins, designated IgG, IgA, IgM, IgD and IgE, which are defined by differences in the constant region of H chains. L chains are divided into kappa or lambda classifications based on structural antigenic differences. All classes of immunoglobulins have been found on the cell surface of B lymphocytes where they function as antigen receptors to elicit antigen-dependent proliferation and secretion of antigen specific soluble circulating antibodies. |
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| Catalog # | K0080-06B |
| Applications | Suitable for use in Flow Cytometry. Other applications not tested. |
| Recommended Dilution | Optimal dilutions to be determined by the researcher. |
| Flow Cytometry Protocol | 1. 5x10e5 ficoll prepared human peripheral white blood cells were preincubated ~5 minutes with 20ul of 250ug/ml human Ig after which they were incubated 45 minutes on ice with 80ml of K0080-06B at 10ug/ml. |
| 2. Cells were washed twice and incubated with 2º reagent Streptavidin/R-PE (S7973-88P) after which they were washed three times, fixed and analyzed using a BD FACS Calibur. |
| 3. Cells stained positive with a mean shift of 2.17 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. |
| 4. Binding was 99% blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml anti-Kappa antibody (K0080-06). |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 7H201 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with Biotin. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Human B lymphoma cells |
| Specificity | Recognizes cell surface kappa on human B cells. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
