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You are here:Home » Antibodies » Abs to IgG Kappa Conjugates » Anti -Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809) (PE)

Anti -Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa
Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km,
MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809) (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified FC
Useful in the identification of leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Immunoglobulin bearing Kappa light chains are present on about 60% of normal B lymphocytes.
Catalog #K0090-08
Fusion Partners Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Recommended Negative Controls Mouse IgG2a Negative Control: RPE
ApplicationsFlow Cytometry: Neat
Optimal working dilutions to be determined by researcher.
Flow Cytometry Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H2575
HostMouse
SourceRat
Concentration~100 Tests/1ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 0.1% sodium azide, 1% BSA. Conjugated to R. Phycoerythrin (RPE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
PurityPurified by Ion Exchange chromatography.
ImmunogenRat F(ab')2 fragment of rat IgG.
SpecificityRecognizes a determinant on rat kappa chains and shows a marked preference for binding to the Igka allotype which is present in AGUS, AO, AUG, BN, LEW, LOU, PVG and WAG rat strains but not in AC1, DA, F344, MSU or SHR.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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