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You are here:Home » Antibodies » Abs to IgG Kappa » Anti -Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809)

Anti -Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa
Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km,
MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC IF
Useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Immunoglobulin bearing Kappa light chains are present on about 60% of normal B lymphocytes.
Catalog #K0090-15
ApplicationsSuitable for use in indirect immunofluorescence and Flow Cytometry. Other applications not tested.
Recommended DilutionIndirect Immunofluorescence/FC: 1ug/1x10e6 cells or 100ul peripheral blood.
Optimal dilution to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F66
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography.
ImmunogenKappa Light Chain
SpecificityRecognizes Kappa light chains of human immunoglobulin and does not react with Lambda light chain or heavy chain. Reacts with CD19 positive lymphocyte (54%) and is negative in other peripheral blood cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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