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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -KPNA2 (Importin Subunit alpha-2, RAG Cohort Protein 1, SRP1-alpha, Karyopherin Subunit alpha-2, RCH1, SRP1)

Anti -KPNA2 (Importin Subunit alpha-2, RAG Cohort Protein 1, SRP1-alpha, Karyopherin
Subunit alpha-2, RCH1, SRP1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IF
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10aa apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins.
Catalog #K0102-06J
ApplicationsSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:32,000
Western Blot: 0.1-0.5ug/ml, observed in Hela lysates on ~55-60kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-QVQDGAPGTFNF, from human KPNA2, at C-terminal (NP_002257.1).
SpecificityRecognizes human KPNA2. Species sequence homology: Canine, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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