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You are here:Home » Antibodies » Abs to Ion Channel » Anti -KCC1 (K, Cl Cotransporter 1)

Anti -KCC1 (K, Cl Cotransporter 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #K0115-03
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blotting 1-10ug/ml for affinity pure or 1:500:1:000 for neat serum using Chemiluminescence technique.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
PurityAffinity purified over the control-peptide Sepharose
ImmunogenAn 21-aa peptide sequence within the cytoplasmic, N-terminus of rat KCC1 (1) was used to generate anti-KCC1. The peptide was coupled with KLH (carrier protein)
SpecificitySequence is 100% conserved in human, rat, mice, porcine and rabbit. No significant sequence homology is seen with other KCCs or CCC. Antibody crossreactivity in various species is not established. We recommend the use of control peptide to establish antibody specificity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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