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You are here:Home » Antibodies » Abs to Ion Channel » Anti -KCC2 (K, Cl Cotransporter 2)

Anti -KCC2 (K, Cl Cotransporter 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. Since cytoplasmic electroneutrality is maintained under normal physiological environment, changes in cellular chloride level is accompanied by total cell solute content. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.
Catalog #K0120-05
The K-Cl cotransporters mediate the coupled movement of K+ and Cl- ions across the cell membranes. This transport process is involved in the regulatory volume decrease in response to cell swelling in red cells and vectorial movement of Cl- ions across the kidney epithelia. The net direction of this transport is out of the cells. At least four isoforms of KCC identified (KCC1, KCC2, KCC3, and KCC4) have been identified and functionally characterized. KCC are predicted to have the same protein structure 12 transmembrane domains with a large extracellular loop with potential N-glycosylation sites, and the cytoplasmic N and C-termini.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500:1:000 using Chemiluminescence technique.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide K0120
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid, 0.05% sodium azide, 40% glycerol.
PuritySerum
ImmunogenAn 18-aa peptide sequence within the cytoplasmic, N-terminus of rat KCC2 (1) was used to generate anti-KCC2 coupled with KLH.
SpecificityRecognizes Rat KCC2. Species sequence homology: mouse and rat-100%. No significant sequence homology is seen with other KCCs or CCC.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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