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You are here:Home » Antibodies » Abs to Ion Channel » Anti -KCC2 (K, Cl Cotransporter 2)

Anti -KCC2 (K, Cl Cotransporter 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IC
KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporters. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
Catalog #K0120-07
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml detected KCC2 in rat brain membrane preparations. Rat brain membrane preparation were resolved by electrophoresis, transferred to nitrocellulose and probed with K0120-07. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunocytochemistry: 1:200 detected KCC2 in MDCK cells as reported by an independent laboratory.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenN-terminal His-tag fusion protein corresponding to residues 932-1043 of rat K+/Cl- Cotransporter (KCC2). (NP_065759)
SpecificityRecognizes rat K+/Cl- Cotransporter (KCC2), Mr ~140kD. An unknown band >200kD may also be detected. Species Crossreactivity: canine. Wide range of species crossreactivity expected due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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