| KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporters. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior. |
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| Catalog # | K0120-07 |
| Applications | Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1ug/ml detected KCC2 in rat brain membrane preparations. Rat brain membrane preparation were resolved by electrophoresis, transferred to nitrocellulose and probed with K0120-07. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. |
| Immunocytochemistry: 1:200 detected KCC2 in MDCK cells as reported by an independent laboratory. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | N-terminal His-tag fusion protein corresponding to residues 932-1043 of rat K+/Cl- Cotransporter (KCC2). |
| Specificity | Recognizes rat K+/Cl- Cotransporter (KCC2), Mr ~140kD. An unknown band >200kD was also detected. Species Crossreactivity: canine. Wide range of species crossreactivity expected due to sequence homology. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
