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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IH

The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
Catalog #	K0138-37
Applications	Suitable for use Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000 detects KDEL-containing proteins in 20ug of HeLa cell lysate
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Purity	Purified by affinity chromatography.
Immunogen	KDEL containing peptide immunogen
Specificity	Recognizes KDEL proteins Grp94, Grp78, PDI and Calreticulin. It may also react with ERp57 and ERp29.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.