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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Keratan Sulfate

Anti -Keratan Sulfate

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Aggrecan is the major proteoglycan of human articular cartilage. The core protein is substituted by a number of keratan sulfate and chondroitin sulfate glycosaminoglycan chains. Whereas chondroitin sulfate is widely distributed throughout the body, keratan sulfate is primarily expressed in cartilage (joints, trachea, intervertebral discs) and cornea.
Catalog #K0197-02
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: <1ug/ml
Western Blot: 1ug/ml
Immunohistochemistry: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No4B3/D10
HostMouse
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 50mM Tris, pH 7.4. Reconstitute with 10ul or 100ul sterile ddH2O.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified human aggrecan
SpecificityRecognizes human Keratan-Sulfate. Preliminary results, based on western Blot of keratan sulfate from different sources, indicate specificity for keratan sulfate from articular cartilage. Minimal if any crossreactivity with keratan sulfate from intervertebral disc. This is in contrast to the epitope recognized by K0197, which recognizes a widely distributed keratan sulfate epitope. Well suited for detecting keratan sulfate fragments released during human inflammatory or degenerative joint diseases into synovial fluid and serum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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