| The polyclonal antibodies react with keratin of most mammals and birds. A positive reaction is obtained with all stratified squamous epithelia and in epidermal appendages such as hairfollicles and sebaceous glands. Also the epithelia of the urinary tract, stomach, small and large intestine, uterine endometrium, bile ducts, prostate, kidney collecting tubules, thymic reticulum cells, mesothelium and to a lesser extent hepatocytes, adenoncarcinomas, transitional cell carcinomas and anaplastic carcinomas can be identified by this antiserum. Renal cell adenocarcinomas and small cell anaplastic lung tumors may remain negative, when examined in paraffin sections. |
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| Hepatocytes and pancreatic acini show a positive staining. In immunohistochemistry nearly all cultured epithelial cell are positive, all non-epthilial cells are negative. |
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| Catalog # | K0199-03E |
| Applications | Suitable for use in Immunohistochemistry. Other application not tested. |
| Recommended Dilution | Immunohistochemistry (Frozen): 1:50 using the ABC method. Fixation for 3 minutes with 1% formaldehyde is recommended. Dilute immediately before use with PBS or Tris. |
| Immunohistochemistry (Frozen and paraffin): 1:50 using the ABC method. For formaldehyde fixed paraffin embedded tissue sections proteolytic pretreatment with pronase or trypsine is recommended. Dilute immediately before use with PBS or Tris. |
| Optimal dilutions to be determined by the researcher. |
| Positive control | Squamous cell carcinoma, skin, cervix |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Concentration | As reported |
| Form | Supplied as a liquid. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Keratin extracted from human skin. Cellular Localization: Cytoplasma |
| Specificity | Recognizes human Keratin. Species Crossreactivity: Most mammals and birds |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
