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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 7 (BSA & Azide Free)

Anti -Keratin 7 (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
Cytokeratin 7 is a basic cytokeratin which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Catalog #K0199-07AX
MW of Antigen 54kD
Epitope Not determined
Applications and Recommended DilutionsImmunohistochemistry (Formalin/paraffin) (Use Ab at 1-2ug/ml for 30 min at RT) * (Immunohistochemical staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with trypsin (Cat. #AP-9008) at 1mg/ml Tris-HCl (or PBS), pH 7.4 for 10-15 min at RT) The optimal dilution for a specific application should be determined by the investigator.
Positive Control Ovarian carcinoma
Cellular Localization Cytoplasmic
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F230
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See K0199-07A
PurityPurified by Protein G affinity chromatography.
ImmunogenOTN II ovarian carcinoma cell line.
SpecificityRecognizes Keratin 7. Species Crossreactivity: human. Does not react with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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