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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 7 (Keratin Type II Cytoskeletal 7, Keratin-7, K7, KRT7, Cytokeratin-7, CK7, CK-7, K2C7, MGC3625, MGC129731, Sarcolectin, SCL, Type-II Keratin Kb7)

Anti -Keratin 7 (Keratin Type II Cytoskeletal 7, Keratin-7, K7, KRT7, Cytokeratin-7, CK7,
CK-7, K2C7, MGC3625, MGC129731, Sarcolectin, SCL, Type-II Keratin Kb7)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
The antibody is a useful marker for histopathology. It can be used to distinguish between ovarian carcinomas and colon carcinomas.
Catalog #K0199-07C
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: Stains a 54kD protein band of cytoskeletal proteins from cell lines in one and two dimensional blots.
Immunohistochemistry (Paraffin): 1:25-1:50. Fix with 4% formalin or with Bouin solution. Pretreat with 0.1% pronase, 0.1% trypsin in PBS or with 0.5% pepsin in 0.01N HCl.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Recommended DilutionThe antibody can be used on paraffin embedded human tissue, fixed with 4% formalin or with Bouin solution. It is advised to pretreat the tissue with 0.1% pronase, 0.1% trypsin in PBS or with 0.5% pepsin in 0.01 N HCl. Advised working dilution: 1:25-1:50.
Clone TypeMonoclonal
IsotypeIgG1
Clone NoOVTL12/30
HostMouse
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenKeratin 7
SpecificityRecognizes human Keratin 7.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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