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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 8,18

Anti -Keratin 8,18

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18 (type A; acidic subfamily of low molecular weight keratins).
Catalog #K0199-08E
MW of Antigen 52.5kD (keratin 8) and 45kD (Keratin 18)
Applications and Recommended DilutionsImmunohistochemistry (Formalin/paraffin) (Ab 1-2ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Positive Control Skin or Breast carcinoma
Cellular Localization Cytoplasmic
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F237
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See K0199-08EX
PurityPurified by Protein G affinity chromatography.
ImmunogenSemi-purified human cytokeratin preparation
SpecificityRecognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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