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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH

Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18 (type A; acidic subfamily of low molecular weight keratins).
Catalog #	K0199-08EX
MW of Antigen	52.5kD (keratin 8) and 45kD (Keratin 18)
Applications and Recommended Dilutions	Immunohistochemistry (Formalin/paraffin) (Ab 1-2ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Positive Control	Skin or Breast carcinoma
Cellular Localization	Cytoplasmic
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3F237
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See K0199-08E
Purity	Purified by Protein G affinity chromatography.
Immunogen	Semi-purified human cytokeratin preparation
Specificity	Recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. Species Crossreactivity: human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.