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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 8, phosphorylated, Ser73

Anti -Keratin 8, phosphorylated, Ser73

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4).
Catalog #K0199-08G
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:10,000
Immunohistochemistry: 1:500 (antigen retrieval required)
Immunocytochemistry: 1:250
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k242
HostRabbit
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in 50mM Tris-Glycine pH 7.4. 0.15M NaCl, 40% glycerol, 0.01% sodium azide.
PuritySupernatant
ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8
SpecificityRecognizes human Cytokeratin 8 phosphorylated on serine 73.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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