Anti -Keratin 8 (Keratin Type II Cytoskeletal 8, Keratin-8, K8, KRT8, CARD2, Cytokeratin-8, CK8, CK-8, CYK8, K2C8, KO, Type-II Keratin Kb8)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IH IC |
|
| Catalog # | K0199-08J | | Applications | Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (Frozen, paraffin): In frozen section, reacts with all simple epithelia derived structures. In paraffin embedded tissues a FKFM-fixation (40% formalin, acetic acid and ddH2O 2:1:17 with 8.5g/L sodium chloride) should be used. Methacarn and formalin fixatives produce less satisfactory results. Protease digestion is beneficial for the result. | | Immunocytochemistry: Strongly reacts with methanol/acetone fixed cells of epithelial origin. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | C-51 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Keratin 8 isolated from HeLa cells | | Specificity | Recognizes the 52.5kD human keratin 8. Species Crossreactivity: Sheep, porcine, bovine. Does not crossreact with canine, rabbit, rat, mouse, chicken or Xenopus. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Keratin 8 (Keratin Type II Cytoskeletal 8, Keratin-8, K8, KRT8, CARD2, Cytokeratin-8, CK8, CK-8, CYK8, K2C8, KO, Type-II Keratin Kb8) |
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