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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 8 (Keratin Type II Cytoskeletal 8, Keratin-8, K8, KRT8, CARD2, Cytokeratin-8, CK8, CK-8, CYK8, K2C8, KO, Type-II Keratin Kb8)

Anti -Keratin 8 (Keratin Type II Cytoskeletal 8, Keratin-8, K8, KRT8, CARD2,
Cytokeratin-8, CK8, CK-8, CYK8, K2C8, KO, Type-II Keratin Kb8)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC
Catalog #K0199-08J
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Frozen, paraffin): In frozen section, reacts with all simple epithelia derived structures. In paraffin embedded tissues a FKFM-fixation (40% formalin, acetic acid and ddH2O 2:1:17 with 8.5g/L sodium chloride) should be used. Methacarn and formalin fixatives produce less satisfactory results. Protease digestion is beneficial for the result.
Immunocytochemistry: Strongly reacts with methanol/acetone fixed cells of epithelial origin.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone NoC-51
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenKeratin 8 isolated from HeLa cells
SpecificityRecognizes the 52.5kD human keratin 8. Species Crossreactivity: Sheep, porcine, bovine. Does not crossreact with canine, rabbit, rat, mouse, chicken or Xenopus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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