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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -KRT8 (Keratin, Type II Cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, CYK8, Type-II Keratin Kb8)

Anti -KRT8 (Keratin, Type II Cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, CYK8,
Type-II Keratin Kb8)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH
Keratin-8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin-8 is primarily found in the non-squamous epithelia, while present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
Catalog #K0199-08S
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 1:100 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlSkin, colon, lung, breast carcinomas
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa1-483 of human Keratin-8.
SpecificityRecognizes human Keratin-8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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