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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP IH

Catalog #	K0199-10
Applications	Suitable for use in Immunohistochemistry (formalin/paraffin) and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution	Immunoprecipitation: 2ug antibody/mg protein lysate. Use Protein G.
	Immunohistochemistry (formalin-fixed, paraffin embedded tissues): 0.5-1ug/ml for 30 mins. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	0.N.351
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Keratin preparation from a human carcinoma.
Specificity	Recognizes keratin 8 at 52.5kDa. The epitope maps between residues 343-357 of the protein. Species Reactivity: Human. Does not react with Rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.