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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 8

Anti -Keratin 8

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH
Catalog #K0199-10
ApplicationsSuitable for use in Immunohistochemistry (formalin/paraffin) and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionImmunoprecipitation: 2ug antibody/mg protein lysate. Use Protein G.
Immunohistochemistry (formalin-fixed, paraffin embedded tissues): 0.5-1ug/ml for 30 mins. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.351
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenKeratin preparation from a human carcinoma.
SpecificityRecognizes keratin 8 at 52.5kDa. The epitope maps between residues 343-357 of the protein. Species Reactivity: Human. Does not react with Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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