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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 9 (Keratin Type I Cytoskeletal 9, Keratin-9, K9, KRT9, Cytokeratin-9, CK-9, EPPK)

Anti -Keratin 9 (Keratin Type I Cytoskeletal 9, Keratin-9, K9, KRT9, Cytokeratin-9, CK-9,
EPPK)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH
The antibody reagent represents an excellent marker to study palmoplantar epidermal distribution and differentiation. Specifically reactive in the middle/upper suprabasal layers (stratum spinosum/granulosum) of the epidermis of palm and sole. Keratin 9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). Keratin 9 has not been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include muscle, liver and duodenum. The antibody cocktail is an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.
Catalog #K0199-10W
ApplicationsSuitable for use in Western Blot, Immunoflourescence Microscopy and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunoflourescence Microscopy: 1:10
Immunohistochemistry (Frozen, paraffin): 1:20 using the PAP/ABC method. Suitable for paraffin when using the microwave method and in some cases protease digestion.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1, IgG3
Clone NoKs 9.70 and Ks 9.216
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid, 0.02% sodium azide.
PuritySupernatant
ImmunogenSynthetic peptides aa450-477 and N-terminal aa4-28 of human Keratin 9 (MW 62,129)
SpecificityRecognizes human Keratin 9.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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