| Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18. |
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| Catalog # | K0199-10X |
| Applications | Suitable for use in Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilution | Immunoprecipitation (Use Protein G): 2ug/mg protein lystate. |
| Immunohistochemistry (formalin/paraffin) | 0.5-1ug/ml for 30 at RT. |
| Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. |
| Optimal dilutions to be determined by the researcher. |
| Postive Control | MCF-7 pr A431 cells, skin, colon, lung or breast carcinoma |
| Cellular Localization | Cytoplasmic |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 0.N.351 |
| Host | Mouse |
| Source | Human |
| Concentration | 1mg/ml |
| Form | Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. |
| Also available with BSA and azide. See K0199-10X. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Keratin preparation from a human carcinoma. |
| Specificity | Recognizes keratin 8 at 52.5kDa. The epitope maps between residues 343-357 of the protein. |
| Species Crossreactivity | Human. Does not react with Rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
