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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IF

Keratin 10 (Cytokeratin 10, CK 10) is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK 10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. Expression of CK 10 is related to maturation of malignant keratinocytes, being preferentially detected in more differentiated parts.
Catalog #	K0199-12
Applications	Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Western Blot: 1-2ug/ml for 2 hours at RT
	Immunohistochemistry (formalin/paraffin): 0.5-1ug/ml for 30 minutes at RT.
	Optimal dilutions to be determined by the researcher.
Positive Control	A431 and skin cells
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.354
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide at 1mg/ml. See K0199-12X.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Cytoskeletal preparation extracted from human ectocervical epithelium. Cellular Localization: Cytoplasmic.
Specificity	Recognizes human Keratin 10 at 56.5kD. Species Crossreactivity: rat, canine and feline.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.