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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IF

Keratin 10 (CK 10) is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK 10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. Expression of CK 10 is related to maturation of malignant keratinocytes, being preferentially detected in more differentiated parts.
Catalog #	K0199-12X
Applications	Immunofluorescence, Western Blotting, Immunohistochemistry.
Recommended Dilution	Western Blotting: 1ug/ml
	Immunohistochemistry: 2-4ug/ml. Frozen & formalin/paraffin. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
	Optimal dilutions to be determined by the researcher.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.354
Host	Mouse
Source	Human
Concentration	1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available with BSA and azide. See K0199-12.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Cytoskeletal preparation extracted from human ectocervical epithelium.
Specificity	Recognizes keratin 10 at 56.5kD. Species crossreactivity: Human, rat, canine and feline.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.