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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 10 (BSA & Azide Free)

Anti -Keratin 10 (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Keratin 10 (CK 10) is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK 10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. Expression of CK 10 is related to maturation of malignant keratinocytes, being preferentially detected in more differentiated parts.
Catalog #K0199-12X
Applications Immunofluorescence, Western Blotting, Immunohistochemistry.
Recommended DilutionWestern Blotting: 1ug/ml
Immunohistochemistry: 2-4ug/ml. Frozen & formalin/paraffin. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilutions to be determined by the researcher.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.354
HostMouse
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available with BSA and azide. See K0199-12.
PurityPurified by Protein G affinity chromatography.
ImmunogenCytoskeletal preparation extracted from human ectocervical epithelium.
SpecificityRecognizes keratin 10 at 56.5kD. Species crossreactivity: Human, rat, canine and feline.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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