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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E B IH

Keratin 14 belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5. Keratin 14 has been studied as a prognostic marker in breast cancer.
Catalog #	K0199-17B
Applications	Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	Western Blot: 1ug/ml for 2 hours at RT. Detects a band at ~50kD.
	Immunohistochemistry (Formalin/paraffin): 1:400-1:800 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
	Optimal dilutions to be determined by the researcher.
Positive Control	A431 cells; Skin or Squamous cell carcinoma
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
Purity	Purified
Immunogen	A synthetic peptide derived from C-terminal of human Keratin 14.
Cellular Localization	Cytoplasmic
Specificity	Recognizes human Keratin 14.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.