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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Keratin 14 belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5. Keratin 14 has been studied as a prognostic marker in breast cancer.
Catalog #	K0199-17X
	The antibody distinguishes stratified epithelial cells from simple epithelial cells and has been reported useful in the identification of squamous cell carcinomas.
Applications	Suitable for use in Immunohistochemistry (frozen & formalin/paraffin) and Western Blot. Other applications not tested.
Recommended Dilution	Immunohistochemistry (formalin/paraffin): 1-2ug/ml for 30 min. at RT.. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min. followed by cooling at RT for 20 min.
Western Blot	1-2ug/ml for 2 hours at RT.
	Optimal dilutions to be determined by the researcher.
Positive Control	A431 cells. Skin or squamous cell carcinoma.
Cellular Localization	Cytoplasmic
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG3
Clone No	0.N.359
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4.
Also available with BSA and azide-use cat # K0199-17.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Synthetic peptide of 15 amino acid residues from the C-terminus of human keratin 14.
Specificity	Recognizes keratin 14 at 50kD. Species Crossreactivity: Human and Rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.