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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 16

Anti -Keratin 16

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified IH IF
Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating.
Catalog #K0199-19
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry (formalin/paraffin). Other applications have not been tested.
Recommended DilutionImmunohistochemistry: 1-2ug/ml for 30 min. at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positve ControlSquamous cell carcinoma
Cellular LocalizationCytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.361
HostMouse
SourceHuman
Concentration200ug/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.
Also available without BSA and azide.
PurityPurified by ammonium sulfate precipitation.
ImmunogenSynthetic peptide corresponding to residues from the C-terminus of human cytokeratin 16
SpecificityRecognizes keratin 16 at 48kD.
Species crossreactivityHuman. Does not react with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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