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You are here:Home » Antibodies » Antibodies-Keratin, Cytokeratin » Anti -Keratin 16 (BSA & Azide Free)

Anti -Keratin 16 (BSA & Azide Free)


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Clone Host Grade Applications
Monoclonal Mouse Highly Purified IH IF
Keratins 6 and 16 are exprssed in keratinocytes, which are undergoing rapid turnover in the suprabasal region. Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. A relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that tumor cells Are not proliferating.
Catalog #K0199-19X
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry (frozen and formalin/paraffin). Other applications have not been tested.
Recommended DilutionImmunohistochemistry: 1-2ug/ml
Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlSquamous cell carcinoma
Cellular LocalizationCytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No0.N.361
FormSupplied as a liquid in 10mM PBS, pH 7.4.
Also available with BSA and azide-use Cat # K0199-19.
PurityPurified by ammonium sulfate precipitation.
ImmunogenSynthetic peptide corresponding to residues from the C-terminus of human cytokeratin 16.
SpecificityRecognizes keratin 16 at 48kD. Species crossreactivity: Human. Does not react with rat.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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