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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant IH IF

The type I keratin 17 (K17) shows a peculiar localization in human epithelial appendages including hair follicles, which undergo a growth cycle throughout adult life. Additionally K17 is induced, along with K6 and K16, early after acute injury to human skin. Predominant expression of K17 and the frequent expression of K8 and K19, with little K6/K16 and K1/K10 expression are the characteristic features of basal cell carcinomas (BCC). This expresssion indicates BCC is differentiated towards undifferentiated follicular epithelia, most probably hair bulge cells.
Catalog #	K0199-20
Applications	Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry (frozen & formalin/paraffin): 1:40-1:80 for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
	Optimal dilutions to be determined by the researcher.
Positive Control	Squamous cell carcinoma or skin
Cellular Localization	Cytoplasmic
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b,k
Clone No	0.N.362
Host	Mouse
Source	Rat
Concentration	Not determined
Form	Supplied as a liquid, concentrated tissue culture supernatant, 15mM sodium azide.
Purity	Tissue culture supernatant
Immunogen	Keratin preparation from rat enterocytes.
Specificity	Recognizes keratin 17 at 46kD. Species Crossreactivity: Human and Rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.