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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IF

Keratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins exists in combination with keratin 8. Tissues from the gastrointestinal tract are positive for both keratin 8 and 18 but do not contain keratin 14. Tissues from gastrointestinal tract, respiratory tract, and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for keratin 18.
Catalog #	K0199-21X
Applications	Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry (frozen and formalin/paraffin). Other applications have not been tested.
Recommended Dilution	Western Blot: 1-2ug/ml for 2 hours at room temperature.
	Immunohistochemistry: 1-2ug/ml for 30 minutes at room temperature.
	Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
	Optimal dilutions to be determined by the researcher.
Positive Control	HT29 cells. Skin, breast or lung carcinoma.
Cellular Localization	Cytoplasmic
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.363
Host	Mouse
Source	Human
Concentration	1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4.
Also available with BSA and azide-use cat # K0199-21.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Human breast cancer PMC 42 cells.
Specificity	Recognzes keratin 18 at 45kD.
Species crossreactivity	Human. Does not react with rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.