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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 18 (BSA & Azide Free)

Anti -Keratin 18 (BSA & Azide Free)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Keratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins exists in combination with keratin 8. Tissues from the gastrointestinal tract are positive for both keratin 8 and 18 but do not contain keratin 14. Tissues from gastrointestinal tract, respiratory tract, and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for keratin 18.
Catalog #K0199-21X
ApplicationsSuitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry (frozen and formalin/paraffin). Other applications have not been tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2 hours at room temperature.
Immunohistochemistry: 1-2ug/ml for 30 minutes at room temperature.
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlHT29 cells. Skin, breast or lung carcinoma.
Cellular LocalizationCytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No0.N.363
FormSupplied as a liquid in 10mM PBS, pH 7.4.
Also available with BSA and azide-use cat # K0199-21.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman breast cancer PMC 42 cells.
SpecificityRecognzes keratin 18 at 45kD.
Species crossreactivityHuman. Does not react with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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