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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 18, phoshorylated (Ser33)

Anti -Keratin 18, phoshorylated (Ser33)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Keratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with keratin 8. It was reported that tissues from gastrointestinal tract are postive for both keratin 8 and 18, but do not contain keratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for keratin 18.
Catalog #K0199-22C2
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1,000
Immunohistochemistry:1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Numbern/a
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthesized phosphopeptide derived from human Keratin 18 around the phosphorylation site of serine 33 (A-A-SP-V-Y).
SpecificityRecognizes endogenous levels of Keratin 18 only when phosphorylated at serine 33.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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