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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 18

Anti -Keratin 18

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Serum B IH IC FC IF
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratins heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
Catalog #K0199-22F
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry. Other applications not tested.
Recommended DilutionWestern Blot: 1:2000 extracts from HeLa and A431 cells. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry: 1:500 Paraffin
Immunocytochemistry: 1:200
Flow Cytometry:
Optimal dilutions to be determined by the researcher.
Molecular Weight 46kD
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10A22
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
PuritySerum
ImmunogenHuman PMC-42 breast carcinoma cells.
SpecificityDetects endogenous levels of total keratin 18 protein. The antibody does not cross-react with other keratins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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