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Specifications

Clone Host Grade Applications Monoclonal Mouse Serum B IH IC FC IF

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratins heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
Catalog #	K0199-22F
Applications	Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry. Other applications not tested.
Recommended Dilution	Western Blot: 1:2000 extracts from HeLa and A431 cells. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
	Immunohistochemistry: 1:500 Paraffin
	Immunocytochemistry: 1:200
	Flow Cytometry:
	Optimal dilutions to be determined by the researcher.
Molecular Weight	46kD
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	10A22
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
Purity	Serum
Immunogen	Human PMC-42 breast carcinoma cells.
Specificity	Detects endogenous levels of total keratin 18 protein. The antibody does not cross-react with other keratins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.