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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 19 (KRT19, CK19, CK-19, Cytokeratin-19, K19, K1CS, Keratin, type I cytoskeletal 19, Keratin-19, MGC15366)

Anti -Keratin 19 (KRT19, CK19, CK-19, Cytokeratin-19, K19, K1CS, Keratin, type I
cytoskeletal 19, Keratin-19, MGC15366)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Cytokeratin 19 is a member of type I acidic subfamily of intermediate filaments. it is expressed in various different human tissues except in liver. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis.
Catalog #K0199-23
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT. Staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. (Protease XXIV damages the epitope recognized by this antibody).
Western Blot: 2-4ug/ml for 2 hours at RT.
Optimal dilutions to be determined by the researcher.
Positive ControlLS174T and HT29 cells, skin
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No0.N.365
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See K0199-23X.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman breast cancer MCF-7 cells. Epitope
SpecificityRecognizes the rod domain of human keratin 19 (40kD). Reacts with the MCF-7 cells, which are known to contain keratin 19. It shows no reaction with the cells lacking keratin 19 such as A431 and HeLa. Species Crossreactivity: Weak reactivity with rat, mouse and guinea pig.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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