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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 19 (KRT19, CK19, CK-19, Cytokeratin-19, K19, K1CS, Keratin, type I cytoskeletal 19, Keratin-19, MGC15366) (BSA & Azide Free)

Anti -Keratin 19 (KRT19, CK19, CK-19, Cytokeratin-19, K19, K1CS, Keratin, type I
cytoskeletal 19, Keratin-19, MGC15366) (BSA & Azide Free)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Cytokeratin 19 is a member of type I acidic subfamily of intermediate filaments. it is expressed in various different human tissues except in liver. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis.
Catalog #K0199-23X
ApplicationsSuitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (frozen and formalin/paraffin): 1-2ug/ml, Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 10-15 min at RT; with trypsin at 1mg/ml Tris-HCl (or PBS), pH 7.4 for 10-15 min at RT); or by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min., followed by cooling at RT for 20 min. (Protease XXIV damages the epitope recognized by this antibody).
Western Blot: 2-4ug/ml for 2 hrs at RT.
Optimal dilutions to be determined by the researcher.
Positive ControlLS174T and HT29 cells, skin
Cellular LocalizationCytoplasmic.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No0.N.365
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See K0199-23.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman breast cancer MCF-7 cells. Epitope: aa312-335
SpecificityRecognizes the rod domain of keratin 19 (40kD). Reacts with the MCF-7 cells, which are known to contain keratin 19. It shows no reaction with the cells lacking keratin 19 such as A431 and HeLa. Species Crossreactivity: human. Weak reactivity with rat, mouse and guinea pig.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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