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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 19 (KRT19, CK19, CK-19, Cytokeratin-19, K19, K1CS, Keratin, type I cytoskeletal 19, Keratin-19, MGC15366)

Anti -Keratin 19 (KRT19, CK19, CK-19, Cytokeratin-19, K19, K1CS, Keratin, type I
cytoskeletal 19, Keratin-19, MGC15366)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IF
Keratin 19 is a member of type I acidic subfamily of intermediate filaments. It is expressed in various different human tissues except in liver. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis.
Catalog #K0199-24D
Epitope C-terminus
ApplicationsImmunofluorescence
Western Blot: (2-4ug/ml for 2hrs at RT)
Immunohistochemistry: Formalin/paraffin, 1:100-200 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Positive Control LS174T, Tonsil
Cellular Localization Cytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide derived from C-terminal of human Keratin 19
SpecificityRecognizes Keratin 19. Species Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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