Anti -Keratin 19 (KRT19, CK19, CK-19, Cytokeratin-19, K19, K1CS, Keratin, type I
cytoskeletal 19, Keratin-19, MGC15366)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH |
|
| Cytokeratin 19 is a member of type I acidic subfamily of intermediate filaments. It is expressed in various different human tissues except in liver. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. | | | Catalog # | K0199-24E | | Applications | Western Blot: 2-4ug/ml for 2 hrs at RT | | Immunohistology: Formalin/paraffin, 1:50-1:100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. | | Positive Control | Ls174T and HT29 Cells, Colon ca. | | Cellular Localization | Cytoplasmic | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 5G141 | | Host | Mouse | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Detergent soluble extract of human mammary epithelium. | | Specificity | Recognizes Keratin 19. Species Crossreactivity: Human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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