Anti -Keratin 19, ID (Keratin, Type I Cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19,
K19)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IF |
|
| Intermediate filaments (IFs), together with actin microfilaments and tubulin microtubules, comprise the three major cytoskeletal networks that are found in most eukaryotic cells. Keratins, which are the largest IF protein subgroup, can be divided into the acidic type I (K9- K20) and basic type II (K1-K8) keratins. Typically, epithelial cells express at least one of each type, and the proteins exist in the cells as noncovalent obligate heteropolymers. Keratin networks are highly dynamic and reorganized during cell differentiation, mitosis, and apoptosis, and also play important cytoprotective and structural support roles for the cells. During apoptosis, the epithelial keratins 18 and 19 undergo caspase-mediated proteolysis, leading to the disassembly of the nuclear envelope and cytoplasmic IF networks. K18 and K19 undergo caspase-cleavage at the conserved Asp237 in their rod domains. | | | Catalog # | K0199-24U | | Applications | Suitable for use in Western Blot and Immunofluorescence. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-2ug/ml | | Immunofluorescence: 2-5ug/ml | | Optimal dilutions to be determined by the researcher. | | Positive Control | HT29 cell lysate | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to an internal sequence surrounding aa120 of human keratin-19. | | Specificity | Recognizes human keratin-19. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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