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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IH

CK20 is a Type-I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. CK20 is expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system. CK20 is also present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack in CK20.
Catalog #	K0199-25
Applications	Suitable for Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1-2ug/ml for 2hrs at RT
	Immunohistochemistry: (formalin/ paraffin)1:25-1:50 for 30 min at RT
	Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C.
	Optimal dilutions to be determined by the researcher.
Positive Control	HT29 cells. Colon carcinoma.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	0.N.367
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 0.09% sodium azide.
Purity	Tissue culture supernatant .
Immunogen	Semi-purified human cytokeratin. Molecular Weight of Antigen
Specificity	Recognizes human keratin 20 at 46kD. Species Crossreactivity: Mouse. Does not react with rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.