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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin 20

Anti -Keratin 20

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH
CK20 is a Type-I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. CK20 is expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system. CK20 is also present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack in CK20.
Catalog #K0199-25
ApplicationsSuitable for Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry: (formalin/ paraffin)1:25-1:50 for 30 min at RT
Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C.
Optimal dilutions to be determined by the researcher.
Positive ControlHT29 cells. Colon carcinoma.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No0.N.367
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.09% sodium azide.
PurityTissue culture supernatant .
ImmunogenSemi-purified human cytokeratin. Molecular Weight of Antigen
SpecificityRecognizes human keratin 20 at 46kD. Species Crossreactivity: Mouse. Does not react with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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