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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin, Epithelial, Type II (Basic Keratins)

Anti -Keratin, Epithelial, Type II (Basic Keratins)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH
This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma.
Catalog #K0199-73
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:100 Detects distinct MW's of 52, 54, 56, 58, 59, 64, and 65-67kD with all Ip's lower then 6.0. These MW's are believed to correspond with keratin 8, 7, 6, 5, 4, 3 and 1 & 2.
Immunohistochemistry: 1:150-1:200 for use on formalin-fixed paraffin embedded tissues. Incubate for 30 minutes at 37ºC. Can also be used with unfixed frozen sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone NoAE-3
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid, 0.1% sodium azide.
PurityPurified
ImmunogenKeratin, Epithelial, Type II
SpecificityRecognizes most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. Broadly reactive with almost all epithelial and their neoplasms. Species Crossreactivity: Rabbit, mouse, rat, bovine, chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesKeratin 1, 2, 3, 4, 5, 6, 7, 8


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