Anti -Keratin, Epithelial, Type II (Basic Keratins)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
B IH |
|
| This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma. | | | Catalog # | K0199-73 | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:100 Detects distinct MW's of 52, 54, 56, 58, 59, 64, and 65-67kD with all Ip's lower then 6.0. These MW's are believed to correspond with keratin 8, 7, 6, 5, 4, 3 and 1 & 2. | | Immunohistochemistry: 1:150-1:200 for use on formalin-fixed paraffin embedded tissues. Incubate for 30 minutes at 37ºC. Can also be used with unfixed frozen sections. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | AE-3 | | Host | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a liquid, 0.1% sodium azide. | | Purity | Purified | | Immunogen | Keratin, Epithelial, Type II | | Specificity | Recognizes most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. Broadly reactive with almost all epithelial and their neoplasms. Species Crossreactivity: Rabbit, mouse, rat, bovine, chicken. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Keratin 1, 2, 3, 4, 5, 6, 7, 8 |
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