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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin, HMW (High Molecular Weight Keratin) discontinued see 138562

Anti -Keratin, HMW (High Molecular Weight Keratin) discontinued see 138562


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Clone Host Grade Applications
Monoclonal Mouse Supernatant E IH IF
In normal cells, Ab labels squamous, ductal and other complex epithelia. It reacts with benign small-acinir lesions of the prostate and does not react with hepatocytes, pancreatic acinar cells, proximal renal tubes or endometrial glands. In tumor cells, this antibody is reactive with both squamous and ductal neoplasms and variably with those derived from simple epithelium.
Catalog #K0199-75B
Immunohistology (Formalin/paraffin): (Use Ab at 1:50-1:100 for 60 min at RT) Staining of formalin-fixed tissues REQUIRES digestion with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C or digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C.
Positive Control Squamous cell carcinoma
Cellular Localization Cytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No5G144
ConcentrationNot determined
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
ImmunogenSolubilized keratin extracted from human stratum corneum.
SpecificityRecognizes keratin 1, 5, 10, and 14 in the Moll catalog (MW 68kD, 58kD, 56.5kD, and 50kD), respectively. Ab is a specific marker useful in differential identification of squamous carcinomas from adenocarcinomas and differential diagnosis of benign and malignant tumors of prostatic gland. Species Crossreactivity: Human, Monkey, Equine, and Rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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