| Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI<5.7) and basic (pI>6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kD. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56 and 52kD. Members of acidic and basic subfamilies are found together in pairs. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. This antibody recognizes 65-67, 64, 59, 58, 56 and 52kD. |
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| Catalog # | K0199-76A |
| Applications | Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry (Formalin fixed paraffin embedded): 1:100 for 10min at RT |
| Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 for10min followed by cooling at RT for 20min. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Skin, squamous cell carcinoma |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | SPM161 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Human epidermal keratin protein |
| Specificity | Recognizes human Keratin HMW. Species Crossreactivity: rat and mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
