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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin, HMW (High Molecular Weight Keratin)

Anti -Keratin, HMW (High Molecular Weight Keratin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IF
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI<5.7) and basic (pI>6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kD. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56 and 52kD. Members of acidic and basic subfamilies are found together in pairs. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. This antibody recognizes 65-67, 64, 59, 58, 56 and 52kD.
Catalog #K0199-76A
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 1:100 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 for10min followed by cooling at RT for 20min.
Optimal dilutions to be determined by the researcher.
Positive ControlSkin, squamous cell carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone NoSPM161
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman epidermal keratin protein
SpecificityRecognizes human Keratin HMW. Species Crossreactivity: rat and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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