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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin, HMW (BSA & Azide Free)

Anti -Keratin, HMW (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Applications: Immunofluorescence, Western Blotting, Immunohistochemistry (frozen & formalin/paraffin). Staining of formalin-fixed tissues requires digestion with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C or digestion of tissue sections with pepsin at 1mg/ml in Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C.
Recommended Dilution:
Western Blotting: 1ug/ml
Immunohistochemistry: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Catalog #K0199-76X
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.369
HostMouse
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.
Also available with BSA and azide. See K0199-76X.
PurityPurified by Protein G affinity chromatography.
ImmunogenCytoskeletal fraction prepared from human gingiva by detergent/salt extraction.
SpecificityRecognizes keratin 13, 14, 15 and 16. Species Reactivity: Human and Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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