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Anti -Keratin K71, IRS1 (Inner Root Sheath 1)
Anti -Keratin K71, IRS1 (Inner Root Sheath 1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Guinea pig |
Serum |
B IH |
|
| Insulin Receptor Substrate (IRS-1) is one of the major substrates of Insulin Receptor Kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain-containing proteins (Grb2, p85, SHP-2, Fyn and others) and which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential Ser/Thr phosphorylation sites for kinases, such as PKA, PKC and MAPK. The phosphorylation of Ser 612 and Ser636/639 are mediated by PKA and mTOR pathways, respectively, resulting in an inhibition of insulin signaling in the cell and suggesting a potential mechanism for insulin resistance in some models of obesity. Once phosphorylated by insulin receptor, IRS-1 recruits the downstream components that mediate the insulin signaling. IRS-1 protein contains a conserved pleckstrin homology (PH) domain located at its amino terminus, adjacent to a phospho-tyrosine binding (PTB) domain. | | | Catalog # | K0199-78 | | Applications | Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. | | Recommended Dilutions | Immunohistochemistry (Frozen): 1:100-1:200. Incubation time: 1 hour at RT. | | Western Blot: 1:2000 using ECL. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Guinea pig | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid, 0.09% sodium azide. | | Purity | Serum | | Immunogen | Synthetic peptide corresponding to human keratin K71, C-GGEGRSRGSANDYKDT (formerly also designated keratin K6irs1), conjugated to KLH. | | Specificity | Recognizes human keratin K71 (57.3kD polypeptide; calculated from sequence data). The antiserum stains specifically the Henle, Huxley and cuticle layer of the inner root sheath (irs) of the hair follicle. Although there is a considerable sequence homology of keratin K71 to keratins K5 and K6, no crossreactivity was observed with Western Blot and immunofluorescence microscopy. | | Species Crossreactivity | Mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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