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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH IC IF

Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry (acetone-fixed frozen and alcohol-or formalin-fixed, paraffin-embedded). Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with pepsin at 1mg/ml in Tris-HCl, pH 2.0 for 10-15 min at RT.
Recommended Dilution:
Immunohistochemistry 1:100-1:200
Optimal dilutions to be determined by the researcher.
Catalog #	K0199-88
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.374
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.
Also available without BSA and azide.
Purity	Purified
Immunogen	Lung cancer cell line.
Specificity	Recognizes keratins of both the acidic and basic subfamilies. Species Reactivity: Human, bovine, Rat, Chicken, Snake and Frog; very wide vertebrate crossreactivity.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.