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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B IH

Among the cytoplasmic intermediate filaments (IF) proteins, keratins make up the largest family and are expressed specifically in epithelial cells in a cell-specific manner. Keratins include more than 20 unique gene products (termed K1-K20) that are divided into type I (K9-K20) and type II (K1-K8). Most epithelial cells express at least one type I and one type II keratin as their predominant IF protein complement in an epithelial cell-specific manner. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2 and the type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Catalog #	K0199-88G
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:10,000
	Western Blot: 1:500-1:2000
	Immunohistochemistry (Formalin fixed paraffin embedded): 1:200
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid.
Purity	Ascites
Immunogen	Ni-NTA purified truncated recombinant CK expressed in E. coli strain BL21 (DE3).
Specificity	Recognizes human Keratin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.