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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin, pan multiple clones

Anti -Keratin, pan multiple clones

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IF
Keratins are resolved with two dimensional gel electrophoresis into acidic pH, <5.7, and basic, pH >6.0 subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Catalog #K0199-91
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1-2ug/ml. Frozen & formalin/paraffin. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT, or Pronase E at 1mg/ml PBS for 5 minutes at 37°C.
Optimal dilutions to be determined by the researcher.
Positive ControlSkin
Cellular LocalizationCytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See K0199-91X.
PurityPurified by Protein A/G affinity chromatography.
SpecificityRecognizes human keratins 4, 5, 6, 7, 8, 10, 13, 14, 15, 16, 18 and 19. Differentiates epithelial tumors from non-epithelial tumors.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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