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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH IF

Keratins are resolved with two dimensional gel electrophoresis into acidic pH, <5.7, and basic, pH >6.0 subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Catalog #	K0199-91
Applications	Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1-2ug/ml. Frozen & formalin/paraffin. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT, or Pronase E at 1mg/ml PBS for 5 minutes at 37°C.
	Optimal dilutions to be determined by the researcher.
Positive Control	Skin
Cellular Localization	Cytoplasmic
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	0.N.373
Host	Mouse
Source	Human
Concentration	200ug/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See K0199-91X.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	Keratin, pan, multiple clones
Specificity	Recognizes human keratins 4, 5, 6, 7, 8, 10, 13, 14, 15, 16, 18 and 19. Differentiates epithelial tumors from non-epithelial tumors.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.