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Keratinocyte Growth Factor, Recombinant, Human (KGF, Fibroblast Growth Factor 7, FGF7, FGF-7, Heparin-binding Growth Factor 7, HBGF-7)
Keratinocyte Growth Factor, Recombinant, Human (KGF, Fibroblast Growth Factor 7, FGF7,
FGF-7, Heparin-binding Growth Factor 7, HBGF-7)
Pricing
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| Catalog # | K0201-15E | | Source | Recombinant corresponding to 164aa of human Keratinocyte Growth Factor expressed in E.coli is a single, non-glycosylated, polypeptide chain. | | Molecular Weight | ~19kD | | Biological Activity | The ED50, calculated by the dose-dependent stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake). | | Dimmers and Aggregates | 1% (SDS-PAGE) | | Protein Content | Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.9 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of KGF as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~19kD | | Purity | ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug) | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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