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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen)

Anti -Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation
Related Ki-67 Antigen)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC FC
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumors is strong. Ki67 is routinely used as a marker of cell cycling and proliferation. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin's lymphoma, mammary carcinomas, ovarian tumors and prostate cancer.
Catalog #K1700-09
ApplicationsSuitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500. Detected cell-cycle-associated protein of 345kD and 395kD on 10ug of A431 lysates.
Immunocytochemistry: 5-10ug/ml
Immunohistochemistry: 5-10ug/ml
Optimal dilution determined by the researcher.
Recommended Secondary AntibodiesI1904-02C: IgG, H&L (HRP) Pab Dk x Mo
I1904-03C: IgG, H&L (X-Adsorbed) (HRP) Pab Dk x Mo
Positive ControlTonsil tissue, A431 Cell Lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q476
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenNuclear protein preparation from the human cell line U937.50.
SpecificityRecognizes a cell-cycle-associated protein of 345kD and 395kD identical with the human Ki-67 antigen. Binds to a formalin-resistant epitope of the Ki-67 antigen. Reactivity is confined to the nuclei of proliferating cells and no crossreactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval. Immunohistochemistry labeling of fresh and frozen samples showed identical results with Ki-67 and Ki-S5.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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