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You are here:Home » Antibodies » Antibodies-Cancer Markers » Anti -Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen)

Anti -Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation
Related Ki-67 Antigen)


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Clone Host Grade Applications
Monoclonal Mouse Supernatant IH
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumors is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
Catalog #K1700-10A
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Acetone and formalin fixed, paraffin-embedded sections): Neat. Incubate 60 minutes at RT. Paraffin requires high temperature unmasking.
Optimal dilutions to be determined by the researcher.
Positive ControlTonsil or breast carcinoma
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No8L521
ConcentrationNot Determined
FormSupplied as a liquid in PBS, sodium azide, Proclin 300.
ImmunogenHuman recombinant Ki67 protein containing the C-terminal 340aa. Cellular Localization: Nuclear.
SpecificityRecognizes human Ki-67 nuclear antigen of 345 and 395kD. It recognizes the native as well as recombinant fragments of Ki-67. Ki-67 nuclear antigen is associated with cell proliferation and is found throughout the cell cycle.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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