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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen)

Anti -Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation
Related Ki-67 Antigen)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
The Ki-67 antigen is preferentially expressed during late G1, S, G2 and M phase of the cell cycle. Resting, non-cycling cells (G0 phase) lack the Ki-67 antigen. Constantly proliferating cells (e.g. cell lines) react positively during the entire cell cycle. This specificity for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin’s lymphoma, mammary carcinomas, ovarian tumors and prostate cancer.
Catalog #K1700-10E
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Immunohistochemistry: frozen and paraffin sections. Paraffin sections require boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.4mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide of Human Ki67. (Note
SpecificityRecognizes human Ki67. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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