| Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki67 index and histologically low-grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. |
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| Catalog # | K1700-11A |
| Applications | Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. |
| Recommended Dilutions | Immunofluorescence (IC): 1:100-1:500 |
| Immunohistochemistry (Paraffin): 1:100-1:500 |
| Immunohistochemistry (Frozen): 1:100-1:500 |
| Flow Cytometry: 1:100 |
| Optimal dilution determined by the researcher. |
| Positive Controls | Mouse intestine, pancreas, spleen |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to an internal portion (within residues 1600-1700) of mouse Ki-67 protein. |
| Cellular Localization | Nuclear |
| Specificity | Recognizes mouse Ki-67. Species Crossreactivity: human |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | anti-Antigen identified by monoclonal Ki 67 antibody, anti-Antigen KI-67 antibody, anti-KIA antibody, anti-Proliferation related Ki 67 antigen antibody, anti-ki-67 antibody |
